PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThis antibody detects endogenous levels of Collagen I
Immunogen Typeprotein
Immunogen DescriptionSynthesized peptide derived from human Collagen I. at AA range: 468-542
Target NameCollagen I
Alternative NamesCollagen alpha-2(I) chain (Alpha-2 type I collagen)
Accession No.Swiss-Prot:P08123 NCBI Gene ID:1278
Calculated MW130kd
Concentration1 mg/ml
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
StorageStore at -20˚C
Application Details
Western blotting: 1:500-1:2000
Images
Western Blot analysis of mouse-brain cells using primary antibody diluted at 1:1000(4˚C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25˚C, 1 hour)