PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ApplicationsWB IHC
Species ReactivityHu
SpecificityRet Polyclonal Antibody detects endogenous levels of Ret
Immunogen Typeprotein
Immunogen DescriptionSynthesized peptide derived from Ret at AA range: 191-240
Target NameRet
Alternative NamesProto-oncogene tyrosine-protein kinase receptor Ret (EC 2.7.10.1) (Cadherin family member 12) (Proto-oncogene c-Ret) [Cleaved into: Soluble RET kinase fragment; Extracellular cell-membrane anchored RET cadherin 120 kDa fragment]
Accession No.Swiss-Prot:P07949 NCBI Gene ID:5979
Calculated MW124kd
Concentration1 mg/ml
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
StorageStore at -20˚C
Application Details
Western blotting: 1:500-1:2000 Immunohistochemistry: IHC: 1:100-1:300
Images
Western Blot analysis of HEPG2 Hela cells using Ret Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200