PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ApplicationsWB IHC
Species ReactivityHu
SpecificityE-Selectin Polyclonal Antibody detects endogenous levels of E-Selectin
Immunogen Typeprotein
Immunogen DescriptionSynthesized peptide derived from E-Selectin . at AA range: INTERNAL
Target NameE-Selectin
Alternative Namesselectin E
Accession No.Swiss-Prot:P16581 NCBI Gene ID:6401
Calculated MW66kd
Concentration1 mg/ml
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
StorageStore at -20˚C
Application Details
Western blotting: 1:500-1:2000 Immunohistochemistry: IHC: 1:100-1:300
Images
Western Blot analysis of K562 SH-SY5Y mouse-brain cells using E-Selectin Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200
Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200