PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThis antibody detects endogenous levels of Rb (Acetyl-K873/K874), It doesn't reacte with total protein.
Immunogen Typeprotein
Immunogen DescriptionSynthesized Acetyl peptide derived from human Rb. at AA range: K873/872
Target NameRb (Acetyl-K873/K874)
Alternative NamesRetinoblastoma-associated protein (p105-Rb) (pRb) (Rb) (pp110)
Accession No.Swiss-Prot:P06400 NCBI Gene ID:5925
Calculated MW106kd
Concentration1 mg/ml
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
StorageStore at -20˚C
Application Details
Western blotting: 1:500-1:2000
Images
Western Blot analysis of 1,mouse-heart 3,mouse-brain cells using primary antibody diluted at 1:1000(4˚C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25˚C, 1 hour)
Yes
