PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThis antibody detects endogenous levels of E2F-1 (Acetyl-K125), It doesn't reacte with total protein.
Immunogen Typeprotein
Immunogen DescriptionSynthesized Acetyl peptide derived from human E2F-1. at AA range: K125
Target NameE2F-1 (Acetyl-K125)
Alternative NamesTranscription factor E2F1 (E2F-1) (PBR3) (Retinoblastoma-associated protein 1) (RBAP-1) (Retinoblastoma-binding protein 3) (RBBP-3) (pRB-binding protein E2F-1)
Accession No.Swiss-Prot:Q01094 NCBI Gene ID:1869
Calculated MW70kd
Concentration1 mg/ml
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
StorageStore at -20˚C
Application Details
Western blotting: 1:500-1:2000
Images
Western Blot analysis of 1,mouse-liver 2,hela cells using primary antibody diluted at 1:1000(4˚C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25˚C, 1 hour)