PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThis antibody detects endogenous levels of ACSS1 (Acetyl-K642), It doesn't reacte with total protein.
Immunogen Typeprotein
Immunogen DescriptionSynthesized Acetyl peptide derived from human ACSS1. at AA range: K642
Target NameACSS1 (Acetyl-K642)
Alternative NamesAcetyl-coenzyme A synthetase 2-like, mitochondrial (EC 6.2.1.1) (Acetate--CoA ligase 2) (Acetyl-CoA synthetase 2) (AceCS2) (Acyl-CoA synthetase short-chain family member 1)
Accession No.Swiss-Prot:Q9NUB1 NCBI Gene ID:84532
Calculated MW70kd
Concentration1 mg/ml
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
StorageStore at -20˚C
Application Details
Western blotting: 1:500-1:2000
Images
Western Blot analysis of 1,293T 2,hela cells using primary antibody diluted at 1:1000(4˚C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25˚C, 1 hour)
Yes
