Tau(Phospho-Thr212) Antibody

Description

Product Name Tau(Phospho-Thr212) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of Tau only when phosphorylated at threonine 212.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of threonine 212 (S-R-T(p)-P-S) derived from Human Tau.
Target Name Tau
Modification Phospho-Thr212
Alternative Names MAPT; MTAPT; MTBT1; Neurofibrillary tangle protein; PHF-tau
Accession No. Swiss-Prot: P10636
NCBI Protein: NP _001116538.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 48 62 78 kd
Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from mouse brain tissue using Tau(Phospho-Thr212) Antibody #AB11257(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).

Background

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Gong CX, et al. (2006) J Biomed Biotechnol. 2006: 31825
Zheng-Fischhofer Q, et al. (1998) Eur J Biochem. 252:542

Published Papers

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