a-Synuclein(Phospho-Tyr125) Antibody

Description

Product Name a-Synuclein(Phospho-Tyr125) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IF
Species Reactivity Human
Specificity The antibody detects endogenous level of a-Synuclein only when phosphorylated at tyrosine 125.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 125 (E-A-Y(p)-E-M) derived from Human a-Synuclein.
Target Name a-Synuclein
Modification Phospho-Tyr125
Alternative Names NACP; SYN; SYUA; alpha-synuclein;
Accession No. Swiss-Prot: P37840
NCBI Protein: NP_000336.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 18kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Images

Western blot analysis of extracts from C2C12 cells untreated or treated with Anisomycin using a-Synuclein(Phospho-Tyr125) Antibody #AB11256.
Immunofluorescence staining of methanol-fixed Hela cells using a-Synuclein(Phospho-Tyr125) Antibody #AB11256.

Background

SncA is a member of the synuclein family of structurally related proteins that are prominently expressed in the central nervous system, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and SncA and Snc-Beta inhibit phospholipase D2 selectively. SncA may serve to integrate presynaptic signaling and membrane trafficking. Aggregated SncA proteins form brain lesions that are hallmarks of neurodegenerative synucleinopathies. Defects in SncA play a role in the pathogenesis of Parkinson disease. SncA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer disease. SncA shares 95% sequence homology with rat SncA. Rat SncA is specifically expressed in brain and is associated with synaptosomal membranes in neurons

Takahashi T, et al. J Biol Chem 2003 Oct 24; 278(43): 42225-33
Ahn BH, et al. J Biol Chem 2002 Apr 05; 277(14): 12334-42
Negro A, et al. FASEB J 2002 Feb; 16(2): 210-2
Goldberg, et al. Nat. Cell Biol. 2000; 2, 115-119.

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