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Recombinant Pseudomonas aeruginosa L-ornithine 5-monooxygenase(pvdA)AP73907

Catalog No.	Package Size	Price
AP73907-1	10ug	$105.00
AP73907-2	50ug	$260.00
AP73907-3	100ug	$454.00
AP73907-4	200ug	$735.00
AP73907-5	500ug	$1240.00
AP73907-6	1mg	$1980.00

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Availability: The lead time is currently 7-30 days.

Description

Product Name Recombinant Pseudomonas aeruginosa L-ornithine 5-monooxygenase(pvdA)
Brief Description Recombinant Protein
Host Species E.coli
Target Name pvdA
Alternative Names L-ornithine N(5)-hydroxylase L-ornithine N(5)-oxygenase Pyoverdin biosynthesis protein A
Accession No. Uniprot ID: Q51548
Target Species Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS
SDS-PAGE MW 65.45kDa
Target Length Full Length,1-443aa
Tag Info N-terminal 6xHis-SUMO-tagged
Target Sequence MTQATATAVVHDLIGVGFGPSNIALAIALQERAQAQGALEVLFLDKQGDYRWHGNTLVSQSELQISFLKDLVSLRNPTSPYSFVNYLHKHDRLVDFINLGTFYPCRMEFNDYLRWVASHFQEQSRYGEEVLRIEPMLSAGQVEALRVISRNADGEELVRTTRALVVSPGGTPRIPQVFRALKGDGRVFHHSQYLEHMAKQPCSSGKPMKIAIIGGGQSAAEAFIDLNDSYPSVQADMILRASALKPADDSPFVNEVFAPKFTDLIYSREHAERERLLREYHNTNYSVVDTDLIERIYGVFYRQKVSGIPRHAFRCMTTVERATATAQGIELALRDAGSGELSVETYDAVILATGYERQLHRQLLEPLAEYLGDHEIGRDYRLQTDERCKVAIYAQGFSQASHGLSDTLLSVLPVRAEEISGSLYQHLKPGTAARALHEHALAS
Formulation Tris-based buffer,50% glycerol
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20˚C/-80˚C. The shelf life of lyophilized form is 12 months at -20˚C/-80˚C.

Application Details

Greater than 90% as determined by SDS-PAGE.

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Background

Catalyzes the conversion of L-ornithine to N(5)-hydroxyornithine, the first step in the biosynthesis of all hydroxamate-containing siderophores, such as pyoverdin. Pyoverdin is a hydroxamate siderophore composed of a 6,7-dihydroxyquinoline-containing fluorescent chromophore joined to the N-terminus of a partly cyclic octapeptide (D-Ser-L-Arg-D-Ser-L-N(5)-OH-Orn-L-Lys-L-N(5)-OH-Orn-L-Thr-L-Thr in strain PAO1). Specific for NADPH, which plays a role in stabilization of the C4a-hydroperoxyflavin intermediate.

Regerences

"Cloning and nucleotide sequence of the pvdA gene encoding the pyoverdin biosynthetic enzyme L-ornithine N5-oxygenase in Pseudomonas aeruginosa."Visca P., Ciervo A., Orsi N.J. Bacteriol. 176:1128-1140(1994)

Published Papers

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