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Recombinant Saccharomyces cerevisiae Eukaryotic translation initiation factor 5A-1(HYP2)AP73765

  Catalog No. Package Size Price
AP73765-1 10ug $105.00
AP73765-2 50ug $260.00
AP73765-3 100ug $454.00
AP73765-4 200ug $735.00
AP73765-5 500ug $1240.00
AP73765-6 1mg $1980.00

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Description
  • Product Name Recombinant Saccharomyces cerevisiae Eukaryotic translation initiation factor 5A-1(HYP2)
  • Brief Description Recombinant Protein
  • Host Species E.coli
  • Target Name HYP2
  • Alternative Names Hypusine-containing protein HP2 eIF-4D
  • Accession No. Uniprot ID: P23301
  • Target Species Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker
  • SDS-PAGE MW 32.97kDa
  • Target Length Full Length,2-157aa
  • Tag Info N-terminal 6xHis-SUMO-tagged
  • Target Sequence SDEEHTFETADAGSSATYPMQCSALRKNGFVVIKSRPCKIVDMSTSKTGKHGHAKVHLVAIDIFTGKKLEDLSPSTHNMEVPVVKRNEYQLLDIDDGFLSLMNMDGDTKDDVKAPEGELGDSLQTAFDEGKDLMVTIISAMGEEAAISFKEAARTD
  • Formulation Tris-based buffer,50% glycerol
  • Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20˚C/-80˚C. The shelf life of lyophilized form is 12 months at -20˚C/-80˚C.
Application Details

Greater than 90% as determined by SDS-PAGE.

Images
  • Recombinant Saccharomyces cerevisiae Eukaryotic translation initiation factor 5A-1(HYP2) - Absci


Background
mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Essential for polarized growth, a process necessary for G1/S transition. May mediate large range of effects of the polyamine spermidine in the cell.
Regerences
"Translation initiation factor 5A and its hypusine modification are essential for cell viability in the yeast Saccharomyces cerevisiae." Schnier J., Schwelberger H.G., Smit-Mcbride Z., Kang H.A., Hershey J.W.B.Mol. Cell. Biol. 11:3105-3114(1991)
    Please let us know if you have published research using #AP73765 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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