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Recombinant Saccharomyces cerevisiae Proliferating cell nuclear antigen(POL30)AP73761

  Catalog No. Package Size Price
AP73761-1 10ug $105.00
AP73761-2 50ug $260.00
AP73761-3 100ug $454.00
AP73761-4 200ug $735.00
AP73761-5 500ug $1240.00
AP73761-6 1mg $1980.00

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Description
  • Product Name Recombinant Saccharomyces cerevisiae Proliferating cell nuclear antigen(POL30)
  • Brief Description Recombinant Protein
  • Host Species E.coli
  • Target Name POL30
  • Alternative Names PCNA
  • Accession No. Uniprot ID: P15873
  • Target Species Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker
  • SDS-PAGE MW 44.9kDa
  • Target Length Full Length,1-258aa
  • Tag Info N-terminal 6xHis-SUMO-tagged
  • Target Sequence MLEAKFEEASLFKRIIDGFKDCVQLVNFQCKEDGIIAQAVDDSRVLLVSLEIGVEAFQEYRCDHPVTLGMDLTSLSKILRCGNNTDTLTLIADNTPDSIILLFEDTKKDRIAEYSLKLMDIDADFLKIEELQYDSTLSLPSSEFSKIVRDLSQLSDSINIMITKETIKFVADGDIGSGSVIIKPFVDMEHPETSIKLEMDQPVDLTFGAKYLLDIIKGSSLSDRVGIRLSSEAPALFQFDLKSGFLQFFLAPKFNDEE
  • Formulation Tris-based buffer,50% glycerol
  • Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20˚C/-80˚C. The shelf life of lyophilized form is 12 months at -20˚C/-80˚C.
Application Details

Greater than 90% as determined by SDS-PAGE.

Images
  • Recombinant Saccharomyces cerevisiae Proliferating cell nuclear antigen(POL30) - Absci


Background
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involved in DNA repair.
Regerences
"Molecular cloning, structure and expression of the yeast proliferating cell nuclear antigen gene."Bauer G.A., Burgess P.M.J.Nucleic Acids Res. 18:261-265(1990)
    Please let us know if you have published research using #AP73761 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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