CDC2(Phospho-Tyr15) Antibody

Description

Product Name CDC2(Phospho-Tyr15) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of CDC2 only when phosphorylated at tyrosine 15.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 15 (G-T-Y(p)-G-V) derived from Human CDC2.
Target Name CDC2
Modification Phospho-Tyr15
Alternative Names CDC28; CDC2A; CDK1; Cyclin-dependent kinase 1;
Accession No. Swiss-Prot: P06493
NCBI Protein: NP_001163877.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 34kd
Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with UV(lane 2) using CDC2(Phospho-Tyr15) Antibody #AB11244.
Western blot analysis of extracts from 293 cells, treated with Hydroxyurea or calf intestinal phosphatase (CIP), using CDC2 (Phospho-Tyr15) Antibody #AB11244.
Western blot analysis of extracts from 293 cells, treated with Hydroxyurea or calf intestinal phosphatase (CIP), using CDC2 (Phospho-Tyr15) Antibody #AB11244.

Background

Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.

Y Gu, et al. (1992) EMBO J. 11(11): 3995

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