c-Kit(Phospho-Tyr721) Antibody

Description

Product Name c-Kit(Phospho-Tyr721) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu
Specificity The antibody detects endogenous level of c-Kit only when phosphorylated at tyrosine 721.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 721 (N-E-Y(p)-M-D) derived from Human c-Kit.
Target Name c-Kit
Modification Phospho-Tyr721
Alternative Names KIT , CD117, SCFR, PBT
Accession No. Swiss-Prot: P10721
NCBI Protein: NP_000213.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 145kd
Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from Hela cells untreated or treated with IFN-a using c-Kit(Phospho-Tyr721) Antibody #AB11240.

Background

This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K)

Nocka, K. et al. (1990) EMBO J. 9, 1805-1813.
Hirota, S. et al. (1998) Science 279, 577-580.
Blume-Jensen, P. et al. (2000) Nat. Genet. 24, 157-162.
Sattler, M. et al. (1997) J. Biol. Chem. 272, 10248-10253.
Gommerman, J.L. et al. (1997) J. Biol. Chem. 272, 30519-30525.

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