S6 Ribosomal Protein(Phospho-Ser235) Antibody

Description

Product Name S6 Ribosomal Protein(Phospho-Ser235) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IF
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of S6 Ribosomal protein only when phosphorylated at serine 235.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine 235 (R-L-S(p)-S-L) derived from Human S6 Ribosomal Protein.
Target Name S6 Ribosomal Protein
Modification Phospho-Ser235
Alternative Names NP33; RPS6; RS6
Accession No. Swiss-Prot: P62753
NCBI Protein: NP_001001.2
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 32kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Images

Western blot analysis of extracts from PC12 cells untreated or treated with anisomycin using S6 Ribosomal Protein(Phospho-Ser235) Antibody #AB11232.
Immunofluorescence staining of methanol-fixed Hela cells using S6 Ribosomal Protein(Phospho-Ser235) Antibody #AB11232.

Background

May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.

McBride K, et al. (1998) Mol Cell Biol 18(9): 5073-5081.
Williams AJ, et al. (2003) Plant Physiol 132(4): 2086-2097.
Wilson MA, et al. (1997) Biochem J 325(Pt 1): 217-222.
Arnesen T, et al. (2005) Biochem J 386(Pt 3): 433-443.

Published Papers

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