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Stathmin1(Phospho-Ser25) AntibodyAB11224

  Catalog No. Package Size Price
AB11224-1 50ul $189.00
AB11224-2 100ul $279.00
AB11224-4 25ul $119.00

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Description
  • Product Name Stathmin1(Phospho-Ser25) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of Stathmin 1 only when phosphorylated at serine 25.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of serine 25 (I-L-S(p)-P-R) derived from Human Stathmin 1.
  • Target Name Stathmin1
  • Modification Phospho-Ser25
  • Alternative Names STMN1; STN1; stathmin
  • Accession No. Swiss-Prot: P16949
    NCBI Protein: NP_001138926.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 19kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images
  • Stathmin1(Phospho-Ser25) Antibody - Absci

    Western blot analysis of extracts from Jurkat cells untreated or treated with PMA using Stathmin 1(Phospho-Ser25) Antibody #AB11224.

  • Stathmin1(Phospho-Ser25) Antibody - Absci

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Stathmin 1(Phospho-Ser25) Antibody #AB11224(left) or the same antibody preincubated with blocking peptide(right).

Background
Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear

Boehm M, et al. (2002) EMBO J 21(13): 3390-3401.
Vadlamudi RK, et al. (2005) Mol Cell Biol 25(9): 3726-3736.
Zilfou JT, et al. (2001) Mol Cell Biol 21(12): 3974-3985.
Biernat J, et al. (2002) Mol Biol Cell 13(11): 4013-4028.
    Please let us know if you have published research using #AB11224 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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