CDC25A(Phospho-Tyr59) Antibody

Description

Product Name CDC25A(Phospho-Tyr59) Antibody
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu
Specificity The antibody detects endogenous level of cdc25A only when phosphorylated at tyrosine 59.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 59 (S-D-Y(p)-E-Q) derived from Human CDC25A.
Modification Phospho-Tyr59
Alternative Names CDC25M3, MPIP1
Accession No. Swiss-Prot#: P30304
NCBI Gene ID: 993
NCBI Protein#: NP_001780.2
Target Species CDC25A
Calculated MW 70kd
Concentration 1.0mg/mL
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C

Application Details

Western blotting: 1:500~1:1000

Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from U87 cells, treated with EGF using CDC25A(Phospho-Tyr59) Antibody #AB11598.
Immunohistochemical analysis of a variety of human tumor tissue using CDC25A(Phospho-Tyr59) Antibody #AB11598.

Background

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

Jin J, et al. (2003) Genes 17(24): 3062-74.
Shimuta K, et al. (2002) EMBO J 21(14): 3694-703.

Published Papers

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