PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu Ms
SpecificityRSK2 (Phospho-Tyr529) Antibody detects endogenous levels of RSK2 only when phosphorylated at Tyr529
Immunogen TypePeptide
Immunogen DescriptionA synthesized peptide derived from human RSK2 (Phospho-Tyr529)