PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu Ms Rt
Specificityp21Cip1 (Phospho-Thr57) Antibody detects endogenous levels of p21Cip1 only when phosphorylated at Thr57
Immunogen TypePeptide
Immunogen DescriptionA synthesized peptide derived from human p21Cip1 (Phospho-Thr57)
Target Namep21Cip1
ModificationPhospho-Thr57
Alternative NamesCDKN1A, CDKN1, CIP1, CDK-interacting protein 1, DNA synthesis inhibitor, p21CIP1, MDA6, PIC1, SDI1, WAF1, CAP20, CDK-interaction protein 1, MDA-6, p21