PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityINCENP (Phospho-Thr59) Antibody detects endogenous levels of INCENP only when phosphorylated at Thr59
Immunogen TypePeptide
Immunogen DescriptionA synthesized peptide derived from human INCENP (Phospho-Thr59)
Target NameINCENP
ModificationPhospho-Thr59
Alternative NamesINCENP, Chromosomal passenger protein, Inner centromere protein