PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityMYPT1/2 (Phospho-Ser668/Ser618) Antibody detects endogenous levels of MYPT1/2 only when phosphorylated at Ser668/Ser618
Immunogen TypePeptide
Immunogen DescriptionA synthesized peptide derived from human MYPT1/2 (Phospho-Ser668/Ser618)
Target NameMYPT1/2
ModificationPhospho-Ser668/Ser618
Alternative NamesPPP1R12A, M130, MYPT1, MBS, Myosin binding subunit