PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityEPHB1/2 (Phospho-Tyr778/Tyr780) Antibody detects endogenous levels of EPHB1/2 only when phosphorylated at Tyr778/Tyr780
Immunogen TypePeptide
Immunogen DescriptionA synthesized peptide derived from human EPHB1/2 (Phospho-Tyr778/Tyr780)