PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityChk2 (Phospho-Ser379) Antibody detects endogenous levels of Chk2 only when phosphorylated at Ser379
Immunogen TypePeptide
Immunogen DescriptionA synthesized peptide derived from human Chk2 (Phospho-Ser379)