PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityHtrA2 (Phospho-Ser142) Antibody detects endogenous levels of HtrA2 only when phosphorylated at Ser142
Immunogen TypePeptide
Immunogen DescriptionA synthesized peptide derived from human HtrA2 (Phospho-Ser142)