PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu
SpecificityAKT1 (Phospho-Ser473+Tyr474) Antibody detects endogenous levels of AKT1 only when phosphorylated at Ser473+Tyr474
Immunogen TypePeptide
Immunogen DescriptionA synthesized peptide derived from human AKT1 (Phospho-Ser473+Tyr474)
Target NameAKT1
ModificationPhospho-Ser473+Tyr474
Alternative NamesAKT1, AKT, AKT-1, C-AKT, Pan-AKT, PKB alpha, PKBalpha, Proto-oncogene c-Akt, Protein kinase B, Rac protein kinase alpha, PRKBA, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB-ALPHA, RAC, RAC-ALPHA