PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu
SpecificitySPHK1 (Phospho-Ser225) Antibody detects endogenous levels of SPHK1 only when phosphorylated at Ser225
Immunogen TypePeptide
Immunogen DescriptionA synthesized peptide derived from human SPHK1 (Phospho-Ser225)
Target NameSPHK1
ModificationPhospho-Ser225
Alternative NamesSPHK1, SK1, Sphingosine kinase 1, SPHK, SK 1, SPK 1, Sphingosine kinase type 1, SPK