PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityAtaxin-3 (Phospho-Ser256) Antibody detects endogenous levels of Ataxin-3 only when phosphorylated at Ser256
Immunogen TypePeptide
Immunogen DescriptionA synthesized peptide derived from human Ataxin-3 (Phospho-Ser256)