PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityERK1/2 (Phospho-Thr202+Tyr204/Thr185+Tyr187) Antibody detects endogenous levels of ERK1/2 (Thr202+Tyr204/Thr185+Tyr187) only when phosphorylated at Thr202+Tyr204/Thr185+Tyr187
Immunogen TypePeptide
Immunogen DescriptionA synthesized peptide derived from human ERK1/2 (Phospho-Thr202+Tyr204/Thr185+Tyr187)