PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu Rt
SpecificitySIK (Phospho-Thr182) Antibody detects endogenous levels of SIK only when phosphorylated at Thr182
Immunogen TypePeptide
Immunogen DescriptionA synthesized peptide derived from human SIK (Phospho-Thr182)