PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu
SpecificityAFX1 (Phospho-Thr455) Antibody detects endogenous levels of AFX1 only when phosphorylated at Thr455
Immunogen TypePeptide
Immunogen DescriptionA synthesized peptide derived from human AFX1 (Phospho-Thr455)
Target NameAFX1
ModificationPhospho-Thr455
Alternative NamesFOXO4, AFX, AFX1, Forkhead box O4, Forkhead box protein O4, MLLT7