PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityCDC25A (Phospho-Ser82) Antibody detects endogenous levels of CDC25A only when phosphorylated at Ser82
Immunogen TypePeptide
Immunogen DescriptionA synthesized peptide derived from human CDC25A (Phospho-Ser82)