IkB-e(Phospho-Ser22) Antibody

Description

Product Name IkB-e(Phospho-Ser22) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu Ms
Specificity The antibody detects endogenous level of IkB-e only when phosphorylated at serine 22.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine 22 (I-E-S(p)-L-R) derived from Mouse IkB-ε.
Target Name IkB-e
Modification Phospho-Ser22
Alternative Names I-kappa-B-epsilon; IKBE; IkappaBepsilon; NF-kappa-BIE; NF-kappaB inhibitor epsilon
Accession No. Swiss-Prot: O54910
NCBI Gene ID: 18037
NCBI Protein: NP_004547.2
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 40kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from 293 cells untreated or treated with TNF using IkB-e(Phospho-Ser22) Antibody #AB11213.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-e(Phospho-Ser22) Antibody #AB11213(left) or the same antibody preincubated with blocking peptide(right).

Background

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.

Shirane M, et al. (1999) J Biol Chem; 274(40): 28169-74
Karin, M. and Ben-Neriah, Y. (2000) Annu. Rev. Immunol. ;18, 621-663.
Chen, Z.J. et al. (1996) Cell; 84, 853-862.
Brown, K. et al. (1995) Science; 267, 1485-1488.

Published Papers

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