Tyrosine Hydroxylase(Phospho-Ser40) Antibody

Description

Product Name Tyrosine Hydroxylase(Phospho-Ser40) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications IF
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of Tyrosine Hydroxylase only when phosphorylated at serine 40.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine 40 (R-Q-S(p)-L-I) derived from Human Tyrosine Hydroxylase (TH).
Target Name Tyrosine Hydroxylase
Modification Phospho-Ser40
Alternative Names TY3H; TYH; Tyrosine 3-hydroxylase; tyrosine hydroxylase;
Accession No. Swiss-Prot: P07101
NCBI Protein: NP_000351.2
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 55-60 kd
Immunofluorescence: 1:100~1:200

Images

Immunofluorescence staining of methanol-fixed Hela cells using Tyrosine Hydroxylase(Phospho-Ser40) Antibody #AB11212.

Background

The protein encoded by Tyrosine Hydroxylase is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Vazin T, et al. Stem Cells. 2008 Jun;26(6):1517-25
Pistocchi A, et al. BMC Dev Biol. 2008 Mar 10;8:27
Fukakusa A, et al. J Pharmacol Sci. 2008 Feb;106(2):321-4.

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