SpecificityThe antibody detects C-terminal, internal, and N-terminal His-tag fusion proteins.
Immunogen TypePeptide
Immunogen DescriptionSynthetic Peptide
Target NameHis-Tag
Concentration1mg/ml
FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
StorageStore at -20˚C/1 year
Application Details
Western blotting: 1:500-1:2000 Immunoprecipitation: 1:100 Immunofluorescence: 1:50-1:100
Images
2ug His fusion protein+ Primary antibody dilution at
1) 1:5000
2) 1:10000
IP antibody use: 5ug His Mouse IgG1 per ml Lysate, WB 1: 3000
1) untransfected 293 cell lysate
2) transfected 293 cell lysate with His-tag fusion protein
3) IP (untransfected 293+anti-His mAb+ Protein G agarose)
4) IP (transfected 293+ normal Mouse IgG
IF analysis of 293 cells transfected with a His-tag protein,1:1000 dilution (blue DAPI, red anti-His)