SpecificityThe antibody detects C-terminal, internal, and N-terminal HA-tag fusion proteins.
Immunogen TypePeptide
Immunogen DescriptionSynthetic Peptide
Target NameHA-Tag
Concentration1mg/ml
FormulationPBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
StorageStore at -20˚C/1 year
Application Details
Western blotting: 1:500-1:2000 Immunoprecipitation: 1:100 Immunofluorescence: 1:50-1:100
Images
0.5ug HA fusion protein+ Primary antibody dilution at 1:10000
IP antibody use: 5ug HA Mouse IgG1 per ml Lysate, WB 1: 5000
1) untransfected 293 cell lysate
2) transfected 293 cell lysate with HA-tag fusion protein
3) IP (untransfted 293+anti-HA mAb+Protein G agarose)
4) IP (transfected 293+ normal Mouse IgG+Prote
IF analysis of 293 cells transfected with a HA-tag protein,1:2000 dilution (blue DAPI, red anti-HA)