IP antibody use: 3ug Flag Mouse IgG1 per ml Lysate, WB 1: 5000
1) untransfected 293 cell lysate
2) transfected 293 cell lysate with Myc-Tag fusion protein
3) IP (transfected 293+ normal Mouse IgG+Protein G agarose)
4) IP (transfected 293+anti- C-Myc m
IF analysis of 293 cells transfected with a Myc-tag protein,1:2000 dilution (blue DAPI, red anti-Myc)