PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of FH only when phosphorylated at threonine 236.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 236(T-H-T(p)-Q-D) derived from Human FH.
Target NameFH
ModificationPhospho-Thr236
Alternative NamesFumarase; fumarate hydratase; Fumarate hydratase, mitochondrial; FUMH; HLRCC;
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 54kd
Western blotting: 1:500~1:1000
Images
Western blot analysis of extracts from Thymidine double block-synchronized U2OS cells (G1 phase) using FH3 (Phospho-Thr236) Antibody #11595. Reference: Nat Cell Biol. 2015 Sep;17(9):1158-68.