PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC
Species ReactivityHu
SpecificityThe antibody detects endogenous level of p27Kip1 only when phosphorylated at threonine 187.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 187 (E-Q-T(p)-P-K) derived from Human p27Kip1.
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 27kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Images
Western blot analysis of extracts from Hela cells untreated or treated with EGF, IFN-a using p27Kip1(Phospho-Thr187) Antibody #AB11208.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p27Kip1(Phospho-Thr187) Antibody #AB11208(left) or the same antibody preincubated with blocking peptide(right).
Background
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.
Eguchi H, et al. (2003) Cancer Res; 63(15): 4739-46
Le XF, et al. (2003) J Biol Chem;
Connor MK, et al. (2003)