NRAS/HRAS/KRAS Antibody

Description

Product Name NRAS/HRAS/KRAS Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of total NRAS/HRAS/KRAS protein.
Immunogen Type Peptide
Immunogen Description Peptide sequence around aa.10~60 derived from Human NRAS/HRAS/KRAS .
Target Name NRAS/HRAS/KRAS
Modification Swiss-Prot: P01111/P01112/P01116
NCBI Protein: NP_002515.1 /NP_001123914.1 . /NP_004976.2
Alternative Names N-Ras; HRAS1; K-Ras 2; Ki-Ras; H-Ras-1
Calculated MW 21kd
Concentration 1.0mg/mL
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from HEK293 cells using NRAS/HRAS/KRAS Antibody #AB43477.

Background

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation.

Regerences

Taparowsky E., Shimizu K., Goldfarb M., Wigler M.Cell 34:581-586(1983) Reddy E.P.Science 220:1061-1063(1983) Shimizu K., Birnbaum D., Ruley M.A., Fasano O., Suard Y., Edlund L., Taparowsky E., Goldfarb M., Wigler M. Nature 304:497-500(1983)

Published Papers

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