HDAC2(Phospho-Ser394) Antibody

Description

Product Name HDAC2(Phospho-Ser394) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC IF
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of HDAC2 only when phosphorylated at serine 394.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine 394 (E-D-S(p)-G-D) derived from Human HDAC2.
Target Name HDAC2
Modification Phospho-Ser394
Alternative Names HD2
Accession No. Swiss-Prot: Q92769
NCBI Protein: NP_001518.2
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images

Western blot analysis of extracts from 293 cells untreated or treated with EGF using HDAC2(Phospho-Ser394) Antibody #AB11191.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC2(Phospho-Ser394) Antibody #AB11191(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells showing nuclear staining using HDAC2(Phospho-Ser394) Antibody #AB11191.
Western blot analysis of extracts from HepG2 cells, treated with calf intestinal phosphatase (CIP), using HDAC2 (Phospho-Ser394) Antibody #AB11191.

Background

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes

Tsai SC, et al.(2002)J Biol Chem; 277(35): 31826-33

Published Papers

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