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APP(Phospho-Thr668) AntibodyAB11190

  Catalog No. Package Size Price
AB11190-1 50ul $189.00
AB11190-2 100ul $279.00
AB11190-4 25ul $119.00

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Description
  • Product Name APP(Phospho-Thr668) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of APP only when phosphorylated at threonine 668.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of threonine 668 (A-V-T(p)-P-E) derived from Human APP.
  • Target Name APP
  • Modification Phospho-Thr668
  • Alternative Names AAA; AD1; PN2; ABPP; APPI
  • Accession No. Swiss-Prot: P05067
    NCBI Protein: NP_000475.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 100-140 kd
Western blotting: 1:500~1:1000

Images
  • APP(Phospho-Thr668) Antibody - Absci

    Western blot analysis of extracts from mouse brain tissue using APP(Phospho-668) Antibody #AB11190(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).

  • APP(Phospho-Thr668) Antibody - Absci

    Western blot analysis of extracts from Hela cells, treated with Noc or calf intestinal phosphatase (CIP), using APP (Phospho-Thr668) Antibody #AB11190.

Background

APP encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene.

Hung, A.Y. and Selkoe, D.J. (1994) EMBO J. 13, 534-542.
Suzuki, T. et al. (1994) EMBO J. 13, 1114-1122
Ando, K. et al. (1999) J. Neurosci. 19, 4421-4427.
Iijima, K.I. et al. (2000) J. Neurochem. 75, 1085-1091

    Please let us know if you have published research using #AB11190 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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