Osteopontin Monoclonal Antibody

Description

Product Name Osteopontin Monoclonal Antibody
Host Species Mouse
Clonality Monoclonal
Purification protein G purifed
Applications WB IHC
Species Reactivity Hu
Specificity specific for human Osteopontin denatured and native forms
Immunogen Type protein
Immunogen Description Recombinant Human Osteopontin
Target Name Osteopontin
Alternative Names Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin, BNSP, OPN, PSEC0156
Accession No. Swiss-Prot#: P10451
Calculated MW 34.5kd
Concentration 1.0mg/mL
Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Storage Store at -20˚C

Application Details

Western blotting: 1:500 - 1:1000
Immunohistochemistry: 1:20 - 1:200

Images

All Lanes Mouse anti human OPN monoclonal antibody at at 2 ug/ml Lane 1 mouse kidney tissue lysate Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution Predicted band size : 34.5 kDa Observed band size: 35kDa Additional bands at : 50 kDa. We are unsure as to the identity of this extra band.
Immunohistochemical analysis of paraffin-embedded human colon cancer using #AB42036 at dilution of 1:200.
Immunohistochemical analysis of paraffin-embedded human Kidney tissue using #AB42036 at dilution of 1:200.

Background

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity

Regerences

[1] Kiefer M.C., Bauer D.M., Barr P.J. The cDNA and derived amino acid sequence for human osteopontin. Nucleic Acids Res. 17:3306-3306(1989) [2] Young M.F., Kerr J.M., Termine J.D., Wewer U.M., Wang M.G., McBride O.W., Fisher L.W. cDNA cloning, mRNA dist

Published Papers

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