Rat TGF-β1 ELISA kit

Description

Product Name Rat TGF-β1 ELISA kit
Brief Description ELISA Kit
Applications Solid Phase Sandwich ELISA
Species Reactivity Rat
Specificity Natural and recombinant Rat TGF-β1 Ligand
Crossing Reactivity No significant interference observed with available related molecules.
Target Name Rat TGF-β1

Application Details

Detect Range: 0.06 - 4.0 ng/mL
Sensitivity: 15pg/mL
Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume: 20 uL
Assay Time: 3 hour
Detection method: Colorimetric

Images

Representative standard curve for TGF-β1 ELISA. TGF-β1 was diluted in serial two-fold steps in Sample Diluent.

Product Description

Aluminium pouches with a Microwell Plate coated with antibody to rat TGF-β1 (8x12)
2 vials rat TGF-β1 Standard lyophilized, 2000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-rat TGF-β1 antibody
2 vials Streptavidin-HRP solution
4 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
2 vial 1N HCl
2 vial 1.2 N NaOH/0.5M HEPES
4 pieces Adhesive Films
package insert

Background

Transforming growth factor (TGF), a 'factor' that promoted the transformation of cultured fibroblasts into a tumor-like phenotype, was subsequently found to be more of a tumor suppressor than tumor promoter and to be a mixture of two proteins, TGF-α and TGF-β. These molecules are members of a superfamily that includes TGF-β1 through 5, bone morphogenic proteins, activins and inhibins. It plays a critical role in cellular growth, development, differentiation, proliferation, extracellular matrix (ECM) synthesis and degradation, control of mesenchymal-epithelial interactions during embryogenesis, immune modulation, apoptosis, cell cycle progression, angiogenesis, adhesion and migration and leukocyte chemotaxis.

Originally, TGF-β1 was separated from platelets and later found that TGF-β1 can be expressed in many organizations. Human TGF-β1 is a 25kDa, disulfide-linked, non-glycosylated homodimer. Biological activity of TGF-β1 is regulated by a number of receptors, including receptor I (53-65KD), receptor II (83-110KD), receptor III (250-310KD), receptor-IV (60KD) and receptor V (400KD).

TGF-β1 is the key mediator in the pathophysiology of tissue repair and human fibrogenesis: balance between production and degradation of type I collagen, and fibrosis and scarring in organ and tissue. TGF-β1 exhibits important immunoregulatory features of partially adverse character: TGF-β1 inhibits B and T cell proliferation, differentiation and antibody production as well as maturation and activation of macrophages. TGF-β1 is synthesized, with only a few exceptions, by virtually all cells, and TGF receptors are expressed by all cells. TGF-β affects nearly every physiological process in some way; its systemic and cell-specific activities are too complicated to review here. There are, however, three fundamental activities: TGF-β1 modulates cell proliferation, generally as a suppressor; TGF-β1 enhances the deposition of extracellular matrix through promotion of synthesis and inhibition of degradation; TGF-β1 is immunosuppressive through a variety of mechanisms. The specific action of TGF-β on a particular cell depends on the exact circumstances of that cell's environment.

Regerences

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