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Mouse IL-12p70 ELISA KITEK0500

  Catalog No. Package Size Price
EK0500-1 1x96T $399.00
EK0500-2 5x96T $1799.00
EK0500-3 10x96T $3459.00

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  • Product Name Mouse IL-12p70 ELISA KIT
  • Brief Description ELISA Kit
  • Applications Solid Phase Sandwich ELISA
  • Species Reactivity Mouse
  • Specificity Natural and recombinant Mouse IL-12p70 Ligand
  • Crossing Reactivity No significant interference observed with available related molecules.
  • Target Name Mouse IL-12p70 ELISA kit
Application Details

Detect Range: 0.02 - 1.0 ng/mL
Sensitivity: 7pg/mL
Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume: 20 uL
Assay Time: 3 hour
Detection method: Colorimetric

  • Mouse IL-12p70 ELISA KIT - Absci

    Representative standard curve for IL-12p70 ELISA. IL-12p70 was diluted in serial two-fold steps in Sample Diluent.

Product Description
  • Aluminium pouches with a Microwell Plate coated with monoclonal antibody to mouse IL-12p70 (8X12)
  • 2 vials mouse IL-12p70 Standard lyophilized, 2000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-mouse IL-12p70 antibody
  • 2 vials Streptavidin-HRP solution,
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 3 pieces Adhesive Films
  • package insert

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of a human B-lymphoblastoid cell line (1-3).

Biologically active mouse IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. While the p40 and p35 subunits by themselves do not have IL-12 activity, the p40 homodimer has been shown to bind the IL-12 receptor and is an IL-12 antagonist (4, 5). Mature mouse p35 subunit is composed of 193 amino acid (aa) residues and contains seven cysteines plus one potential N-linked glycosylation site (6). Mature mouse p40 subunit has 313 aa, with 13 cysteines and five potential N-linked glycosylation sites (6). Mouse p35 and p40 subunits show 63% and 72% aa identity, respectively, to the human p35 and p40 subunits (3, 6). Although mouse IL-12 is active on both human and mouse cells, human IL-12 is only active on human cells.

IL-12 has been shown to have multiple effects on T lymphocytes and natural killer (NK) cells. Some of these effects include the induction of IFN-γ and TNF production by T and NK cells, the enhancement of cytotoxic activity of T and NK cells and the stimulation of T and NK cell proliferation. IL-12 has also been shown to be a central mediator of the cell-mediated immune response by promoting Th1 development (7-11).

Cell surface staining for IL-12 on a human monocytic and a mouse macrophage cell line has been reported, suggesting that membrane-associated IL-12 may exist (12). Cells known to produce IL-12 include macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, and keratinocytes. Although a human B cell line has been shown to produce IL-12(2), fresh B cells are apparently not producers of IL-12.


Kobayashi, M. et al. (1989) J. Exp. Med. 170:827.

Stern, A.S. et al. (1990) Proc. Natl. Acad. Sci. USA 87:6808.

Gubler, U. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4143.

Gillessen, S. et al. (1995) Eur. J. Immunol. 25:200.

Ling, P. et al. (1995) J. Immunol. 154:116

Schoenhaut, D.S. et al. (1992) J. Immunol. 148:3433.

Trinchieri, G. (1995) Annu. Rev. Immunol. 13:251.

Pudda, P. et al. (1997) J. Immunol. 159:3490.

Windhagen, A. et al. (1996) J. Immunol. 157:1127.

Mehrotra, P.T. et al. (1998) J. Immunol. 160:2637.

Wolf, S. F. et al. (1994) Stem Cells 12:154.

Fan, X. et al. (1996) Biochem. Biophys. Res. Commun. 225:1063.

    Please let us know if you have published research using #EK0500 so that we can cite your reference.
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    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
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    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
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