Human P-selectin ELISA kitEK0490

• Aluminium pouches with a Microwell Plate coated with antibody to human P-Selectin (8x12)
• 2 vials human P-Selectin Standard lyophilized, 4000 pg/ml upon reconstitution
• 2 vials concentrated Biotin-Conjugate anti-human P-Selectin antibody
• 2 vials Streptavidin-HRP solution
• 4 bottle Standard /sample Diluent
• 1 bottle Biotin-Conjugate antibody Diluent
• 1 bottle Streptavidin-HRP Diluent
• 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
• 1 vial Substrate Solution
• 1 vial Stop Solution
• 4 pieces Adhesive Films
• package insert

P-Selectin (GMP-140, LECAM-3, PADGEM, CD62, CD62P) is a cell surface glycoprotein that plays a critical role in the migration of lymphocytes into tissues (1 - 5). It is found constitutively in a pre-formed state in the Weibel-Palade bodies of endothelial cells and in the alpha granules of platelets (4). This stored P-Selectin is mobilized to the cell surface within minutes in response to a variety of inflammatory or thrombogenic agents (4). The mobilized P-Selectin is apparently present on the cell surface for only a few minutes after which it is recycled to intracellular compartments (4). Additional evidence indicates that transcription of P-Selectin mRNA can be activated in the endothelium by treatment with inflammatory mediators (6). P-Selectin consists of an NH2-terminal lectin type C domain, an EGF-like domain, nine complement control domains, a transmembrane domain, and a short cytoplasmic domain (1,2). Mouse P-Selectin shows a similar organization of functional domains and an overall sequence identity of approximately 73% (5, 6). However, mouse P-Selectin contains only eight complement control domains, suggesting that the absolute number of these domains is not crucial for function (6). The molecular weight predicted from the cDNA for P-Selectin is approximately 86,000 (1, 2). The observed molecular weight on reducing SDS-PAGE,however, is approximately 140,000 (2).

Evidence indicates that P-Selectin is involved in the adhesion of myeloid cells, as well as B cells and a subset of T cells, to activated endothelium (4). P-Selectin is also involved in the adhesion of platelets to monocytes and neutrophils, playing a central role in neutrophil accumulation within thrombi (4). The adhesion of leukocytes and neutrophils to the endothelium is initiated by weak interactions that produce a characteristic

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