Human PBEF/ Visfatin ELISA kitEK0483

• Aluminium pouches with a Microwell Plate coated with antibody to human PBEF/Visfatin (8X12)
• 2 vials human PBEF/Visfatin Standard lyophilized, 50 ng/ml upon reconstitution
• 2 vials concentrated Biotin-Conjugate anti-human PBEF/Visfatin antibody
• 2 vials Streptavidin-HRP solution
• 4 bottle Standard /sample Diluent
• 1 bottle Biotin-Conjugate antibody Diluent
• 1 bottle Streptavidin-HRP Diluent
• 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
• 1 vial Substrate Solution
• 1 vial Stop Solution
• 4 pieces Adhesive Films
• package insert

PBEF (preB cell colonyenhancing factor? also visfatin and nicotinamide phosphoribosyltransferase) is a 52 kDa member of the NAPRTase family of molecules. It functions both intracellularly and extracellularly, where it participates in NAD synthesis and insulin receptor activation, respectively. Human PBEF is 491 amino acids in length and contains no signal sequence. There is at least one alternative splice form that shows a 5 aa substitution for the Cterminal 128 amino acids (aa 364491). Over aa 27491, human PBEF shares 96%, 97%, and 96% aa identity with mouse, porcine, and canine PBEF, respectively.

Kitani,T,Okuno,S.,Fujisawa,H(2003) Growth phase-dependent changes in the subcellular Iocalization of per-B-cell colony-enhancing factor,FEBSLett,544.,74-78.

Martin,P.R.,Shea,R.J.,Mulks,M.H.(2001) Identication of a plasmid-encoded gene from Haemophilus ducreyi which confers NAD independence,J., Bacteriol,183,1168-1174.

Ognjanovic,S.,Bao,S.,Yamamoto,S.Y.,Garibay-Tupas,J.,Samal,B.,Bryant-Green wood,G,D,(2001) Genomic organization of the gene coding for human-pre-B-cell colong enhancing factor and expression in human fetal membranes.J.Mol.Endocrinol.26,107-117.

Ye,S,O.,Simon,B,A.,Maloney,J,P.,Zambelliweiner,A.,Gao,L.,Grant,A.,Easley,R,B.,Mcverry,B,J.,Tuder,R,M.,Standiford,T.,Brower,R,G.,Barnes,K,C.,Garcia,J,G.(2005)Pre-B-cell colony-enhancing facto rasapotential novel biomarkerin acute lung injury,Am,J.Respir,Crit.CareMed.171,360-370.

Stephens JM.,Vidal-Puig AJ,(2006) An update on visfatin/pre-B cell colony enhancing factor, an ubiquitously expressed, illusive cytokine that is regulated in obesity, Curr Opin Lipidol,17(2);128-131.