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Human IL-22 ELISA kitEK0477

  Catalog No. Package Size Price
EK0477-1 1x96T $399.00
EK0477-2 5x96T $1799.00
EK0477-3 10x96T $3459.00

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Description
  • Product Name Human IL-22 ELISA kit
  • Brief Description ELISA Kit
  • Applications Solid Phase Sandwich ELISA
  • Species Reactivity Human
  • Specificity Natural and recombinant Human IL-22 Ligand
  • Crossing Reactivity No significant interference observed with available related molecules.
  • Target Name Human IL-22
Application Details

Detect Range: 31.25-2000pg/ml
Sensitivity: 15pg/mL
Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume: 20 uL
Assay Time: 3 hours
Detection method: Colorimetric

Images
  • Human IL-22 ELISA kit - Absci

    Representative standard curve for IL-22 ELISA. IL-22 was diluted in serial two-fold steps in Sample Diluent.

Product Description
  • Aluminium pouches with a Microwell Plate coated with monoclonal antibody to human IL-22 (8X12)
  • 2 vials human IL-22 Standard lyophilized, 2000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-human IL-22 antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert
Background

Interleukin 22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF), is a member of the IL-10 cytokine family. Other members of this family include IL-10, IL-19, IL-20, IL-24, and IL-26 (1). IL-22 was initially identified as a gene induced by IL-9 in mouse T cells and mast cells (2). Human IL-22 cDNA encodes a 179 amino acid (aa) protein with a putative 33 aa signal peptide, sharing approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively (3, 4). Although the related IL-10 is thought to act as a dimer, the crystal structure of IL-22 suggests it may interact with its receptor as a monomer (5). The functional IL-22 receptor is of the class 2 subtype and consists of two receptor subunits,IL-22 R (previously an orphan receptor named CRF2-9) and IL-10 R (previously known as CRF2-4) (6). The IL-10 R chain is shared by IL-10, IL-26, IL-28A, IL-28B, and IL-29 (7, 8).

IL-22 R is expressed primarily in the pancreas, and to a lesser extent, tissues of the gastrointestinal tract, kidney, and skin (7, 9 - 12). A soluble receptor, IL-22 binding protein (IL-22BP), has also been described and may act as an endogenous inhibitor of IL-22 activity (13 - 15). IL-22 has been shown to activate Jak/STAT and MAPK signaling pathways and upregulate the production of acute phase proteins (3, 4, 6, 16 - 18).

IL-22 is produced primarily by activated Th1-type T cells and NK cells (19). Mouse IL-22 expression is induced in various organs upon lipopolysaccharide injection, suggesting that it may be involved in inflammatory responses (3). In humans, this is supported by the observation that IL-22 is produced by synovial fibroblasts and macrophages of rheumatoid arthritis (RA) patients and is capable of inducing pro-inflammatory responses in RA synovial tissues (20). In addition, it stimulates the production of pro-inflammatory cytokines and anti-microbial defensins in human keratinocytes (9, 10). These activities result in epidermal hyperplasia in models of human skin (9).

Regerences

Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.

Dumoutier, L. et al. (2000) J. Immunol. 164:1814.

Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.

Xie, M.H. et al. (2000) J. Biol. Chem. 275:31335.

Nagem, R.A. et al. (2002) Acta Crystallogr. D. Biol. Crystallogr. 58:529.

Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.

Kotenko, S.V. and J.A. Langer. (2004) Int. Immunopharmacol. 4:593.

Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314.

Boniface, K. et al. (2005) J. Immunol. 174:3695.

Wolk, K. et al. (2004) Immunity 21:241.

Gurney, A.L. (2004) Int. Immunopharmacol. 4:669.

Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.

Dumoutier, L. et al. (2001) J. Immunol. 166:7090.Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.

Xu, W. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9511.

Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.

Lejeune, D. et al. (2002) J. Biol. Chem. 277:33676.

Dumoutier, L. et al. (2000) Genes Immun. 1:488.

Wolk, K. et al. (2002) J. Immunol. 168:5397.

Ikeuchi, H. et al. (2005) Arthritis Rheum. 52:1037.9.

    Please let us know if you have published research using #EK0477 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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