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Human IL-21 ELISA kitEK0476

  Catalog No. Package Size Price
EK0476-1 1x96T $399.00
EK0476-2 5x96T $1799.00
EK0476-3 10x96T $3459.00

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Description
  • Product Name Human IL-21 ELISA kit
  • Brief Description ELISA Kit
  • Applications Solid Phase Sandwich ELISA
  • Species Reactivity Human
  • Specificity Natural and recombinant Human IL-21 Ligand
  • Crossing Reactivity No significant interference observed with available related molecules.
  • Target Name Human IL-21
Application Details

Detect Range: 31.25-2000pg/ml
Sensitivity: 15pg/mL
Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume: 20 uL
Assay Time: 3 hours
Detection method: Colorimetric

Images
  • Human IL-21 ELISA kit - Absci

    Representative standard curve for IL-21 ELISA. IL-21 was diluted in serial two-fold steps in Sample Diluent.

Product Description
  • Aluminium pouches with a Microwell Plate coated with antibody to human IL-21 (8x12)
  • 2 vials human IL-21 Standard lyophilized, 1000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-human IL-21 antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert
Background

Interleukin-21(IL-21) and its receptor play an important role in the regulation of the immune system. IL-21R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. The biological effects of IL-21 include induction of differentiation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjuction) with IL-4 of IgG production, and induction of apoptotic effects in native B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction appears to play an important role in B and T cell proliferation after antigen stimulation and NK cell maturation.

Regerences

Parrish-Novak, J. et al. (2000) Nature 408: 57.

Parrish-Novak, J. et al. (2002) J Leukoc Biol 72: 856.

Konforte, D. et al. (2009) J Immunol 182: 1781.

Strengell, M. et al. (2002) J Immunol 169: 3600.

Davis, I.D. et al. (2007) Clin Cancer Res 13: 6926.

    Please let us know if you have published research using #EK0476 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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