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Human Resistin ELISA kitEK0469

  Catalog No. Package Size Price
EK0469-1 1x96T $399.00
EK0469-2 5x96T $1799.00
EK0469-3 10x96T $3459.00

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Description
  • Product Name Human Resistin ELISA kit
  • Brief Description ELISA Kit
  • Applications Solid Phase Sandwich ELISA
  • Species Reactivity Human
  • Specificity Natural and recombinant Human Resistin Ligand
  • Crossing Reactivity No significant interference observed with available related molecules.
  • Target Name Human Resistin
Application Details

Detect Range: 7.8-500pg/ml
Sensitivity: 4pg/mL
Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume: 20 uL
Assay Time: 3 hours
Detection method: Colorimetric

Images
  • Human Resistin ELISA kit - Absci

    Representative standard curve for Resistin ELISA. Resistin was diluted in serial two-fold steps in Sample Diluent.

Product Description
  • Aluminium pouches with a Microwell Plate coated with antibody to human Resistin (8X12)
  • 2 vials human Resistin Standard lyophilized, 1000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-human Resistin antibody
  • 2 vials Streptavidin-HRP solution
  • 4 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert
Background

Resistin, also known as Found In Inflammatory Zone 3 (FIZZ3) or Adipocyte Secreted Factor(ADSF), is a member of a protein family known as the Resistin-like molecules (RELMs). It is perhaps best known for its potential as a link between obesity and the development of insulin resistance (1). Other members of this family include RELM-α/FIZZ1 and RELM-γ, which are described in rodents but as yet have no identified human counterparts, and RELM-β/FIZZ2(2, 3). The Resistin amino acid (aa) sequence contains a putative N-terminal signal sequence and a motif containing 11 cysteine residues, 10 of which are characteristic of the RELM family(1-3). The protein is thought to be secreted as a dimer and potentially exists in higher order molecular structures resulting from interactions between Resistin dimers or other members of the RELM family (4-7). A splice variant in the rat, lacking the signal sequence and localized predominantly to the nucleus, has also been described (8). A large 3' intron is the primary reason that the mouse genomic sequence is 3-fold larger than the corresponding human sequence (9). Mouse and human Resistin share only 53 percent identity at the aa level and exhibit differences in expression patterns (1, 9, 10). In mouse, expression appears primarily in adipose tissues (1). Although some human studies suggest Resistin is expressed by adipose tissues as well, the most significant source appears to be blood mononuclear cells (11-13). In humans, Resistin is also reported to be expressed by pre-adipocytes (14), placenta (15), pancreatic islets (16), and primary leukemia cells (10). A receptor for Resistin has not yet been described.

Resistin acquired initial attention as a potential link between obesity and glucose regulation. Serum levels were shown to increase in diet-induced and genetic forms of obesity in mice (ob/ob and db/db) and decrease in response to insulin sensitizing drugs (TZDs) (1). In addition, function-blocking Resistin antibodies enhanced insulin actions while treatment with recombinant Resistin caused glucose intolerance and insulin resistance (1). Resistin knockout mice exhibit decreased fasting blood glucose levels as a result of reduced hepatic output (17). To establish a physiological role in humans, several studies have examined whether altered circulating Resistin levels are associated with type 2 diabetes, insulin resistance, and/or obesity. Although some demonstrate significant correlations (18-23), others report no correlation (23-28), suggesting that in humans fundamental questions remain regarding Resistin

Regerences

Steppan, C.M. et al. (2001) Nature 409:307.

Steppan, C.M. et al. (2001) Proc. Natl. Acad. Sci. USA 98:502.

Gerstmayer, B. et al. (2003) Genomics 81:588.

Raghu, P. et al. (2004) Biochem. Biophys. Res. Commun. 313:642.

Banerjee, R.R. and M.A. Lazar (2001) J. Biol. Chem. 276:25970.

Chen, J. et al. (2002) J. Endocrinol. 175:499.

Aruna, B. et al. (2003) Biochemistry 42:10554.

Del Arco, A. et al. (2003) FEBS Lett. 555:243.

Ghosh, S. et al. (2003) Gene 305:27.

Yang, R.Z. et al. (2003) Biochem. Biophys. Res. Commun. 310:927.

McTernan, P.G. et al. (2002) J. Clin. Endocrinol. Metab. 87:2407.

Nagaev, I. and U. Smith (2001) Biochem. Biophys. Res. Commun. 285:561.

Savage, D.B. et al. (2001) Diabetes 50:2199.

Janke, J. et al. (2002) Obes. Res. 10:1.

Yura, S. et al. (2003) J. Clin. Endocrinol. Metab. 88:1394.

Minn, A.H. et al. (2003) Biochem. Biophys. Res. Commun. 310:641.

Banerjee, R.R. et al. (2004) Science 303:1195.

McTernan, P.G. et al. (2003) J. Clin. Endocrinol. Metab. 88:6098.

Degawa-Yamauchi, M. et al. (2003) J. Clin. Endocrinol. Metab. 88:5452.

Youn, B.S. et al. (2004) J. Clin. Endocrinol. Metab. 89:150.

Fujinami, A. et al. (2004) Clin. Chim. Acta 339:57.

Azuma, K. et al. (2003) Obes. Res. 11:997.

Silha, J.V. et al. (2003) Eur. J. Endocrinol. 149:331.

Fehmann, H.C. and J. Heyn (2002) Horm. Metab. Res. 34:671.

Pfutzner, A. et al. (2003) Clin. Lab. 49:571.

Kielstein, J.T. et al. (2003) Am. J. Kidney Dis. 42:62.

Lee, J.H. et al. (2003) J. Clin. Endocrinol. Metab. 88:4848.

Furuhashi, M. et al. (2003) Clin. Endocrinol. 59:507.

Smith, U. (2002) Obes. Res. 10:61.

Ukkola, O. (2002) Eur. J. Endocrinol. 147:571.

Kaser, S. et al. (2003) Biochem. Biophys. Res. Commun. 309:286.

Kawanami, D. et al. (2004) Biochem. Biophys. Res. Commun. 314:415.

Verma, S. et al. (2003) Circulation 108:736.

    Please let us know if you have published research using #EK0469 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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