Human IL-3 ELISA kitEK0466

• Aluminium pouches with a Microwell Plate coated with antibody to human IL-3 (8x12)
• 2 vials human IL-3 Standard lyophilized, 1000 pg/ml upon reconstitution
• 2 vials concentrated Biotin-Conjugate anti-human IL-3 antibody
• 2 vials Streptavidin-HRP solution
• 1 bottle Standard /sample Diluent
• 1 bottle Biotin-Conjugate antibody Diluent
• 1 bottle Streptavidin-HRP Diluent
• 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
• 1 vial Substrate Solution
• 1 vial Stop Solution
• 4 pieces Adhesive Films
• package insert

Interleukin-3, also known as mast cell growth factor, P-cell stimulating factor, burst promoting activity, multicolony stimulating factor, t h y-1 inducing factor and WEHI-3 growth factor, is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes can also produce IL-3. Mature human IL-3 share only 29% aa sequence identity with murine IL-3. IL-3 activity is highly speciesspecific and human IL-3 does not show activity on murine cells. IL-3 is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.

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