Human GM-CSF ELISA kitEK0464

• Aluminium pouches with a Microwell Plate coated with monoclonal antibody to human GM-CSF (8x12)
• 2 vials human GM-CSF Standard lyophilized, 2000 pg/ml upon reconstitution
• 2 vials concentrated Biotin-Conjugate anti-human GM-CSF monoclonal antibody
• 2 vials Streptavidin-HRP solution
• 1 bottle Standard /sample Diluent
• 1 bottle Biotin-Conjugate antibody Diluent
• 1 bottle Streptavidin-HRP Diluent
• 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
• 1 vial Substrate Solution
• 1 vial Stop Solution
• 3 pieces Adhesive Films
• package insert

Granulocyte macrophage colony stimulating factor (GM-CSF) is a pleiotropic cytokine with multiple effects on hematopoietic cells (1-4). It mobilizes CD34+ progenitor cells into the periphery and stimulates their proliferation, survival and differentiation into neutrophils,monocytes/ macrophages, eosinophils, and myeloid dendritic cells (4-8). On these terminallydifferentiated myeloid cells, GM-CSF is also needed for inducing their effector functions (7-10). In addition, GM-CSF has been shown to stimulate the proliferation and differentiation of the erythroid and megakaryoctye progenitor cells (4).

GM-CSF is produced by a number of different cell types, including keratinocytes, mature and immature NK cells, type II alveolar cells), endothelial cells, monocytes, bone-marrow mesenchymal stem cells, CD4+ and CD8+ T cells, megakaryocytes, B cells, eosinophils, chondrocytes and fibroblasts.

Human GM-CSF cDNA encodes a 144 amino acid (aa) residue precursor protein with a 17 aa putative signal peptide and a 127 aa mature protien (11 - 13). Natural GM-CSF is a monomer that contains both N- and O-linked glycosylation (14). Mature human GM-CSF shares approximately 55%, 63% and 68% aa sequence homology with mouse, rat and canine. GM-CSF, respectively. Human GM-CSF is not biologically active on mouse cells (11), but was reported to have some activity on canine cells (15).

GM-CSF exerts its activity through binding to a high affinity receptor complex consisting of two membrane glycoproteins. The presence of the spliced variants in the heteromeric receptor complex can regulate the functions of the complex (16).

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